Detection of FLT3 Internal Tandem Duplication and D835 Mutations by a Multiplex Polymerase Chain Reaction and Capillary Electrophoresis Assay

Incidence and prognostic value of FLT3 internal tandem duplication and D835 mutations in acute myeloid leukemia.

BACKGROUND AND OBJECTIVES Cytogenetics is the most important prognostic factor in acute myeloid leukemia (AML). However, a high proportion of patients show normal or intermediate-risk karyotypes. In these patients, other determinants could help to identify those with a higher risk of relapse. Recently, internal tandem duplications (ITD) and D835 mutations in FLT3 tyrosine kinase receptor have b...

Simplified capillary electrophoresis detection of the Flt-3 internal tandem duplications and D835 point mutations in acute myeloid leukemia.

Introduction Flt-3 mutations, which include the internal tandem duplications (ITD) and missense mutations at the D835 (aspartic acid) codon,2 are arguably the most common somatic mutations in acute myeloid leukemia (AML). As relapse remains the principal cause of treatment failure for the majority of AML patients, identification of patients with a high risk of relapse would be useful for the in...

FLT3 Internal Tandem Duplication and D835 Mutations in Patients with Acute Lymphoblastic Leukemia and its Clinical Significance

The fms-like tyrosine kinase 3 (FLT3) gene is a member of the class III receptor tyrosine kinase family. Mutations of FLT3 were first described in 1997 and account for the most frequent molecular mutations in acute myeloid leukemia. Currently, there is no published data on FLT3 mutations in Saudi acute lymphoblastic leukemia (ALL) patients. In this retrospective study, we have examined a cohort...

A Diagnostic Multiplex Polymerase Chain Reaction Assay for Ocular Herpetic Infections.

Rapid Detection of Campylobacter jejuni by Polymerase Chain Reaction and Evaluation of its Sensitivity and Specificity

Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was sele...